Quantitative Analysis of Biochemical Constituents in Tulsi (Ocimum sanctum L.)

Tulsi (Ocimum sanctum L.), commonly known as Holy Basil, is an important medicinal plant widely used in traditional Indian medicine. The present study was conducted to evaluate the biochemical composition of Tulsi leaves and assess their medicinal significance. Fresh leaf samples were analysed for various biochemical parameters, including chlorophyll a, chlorophyll b, total chlorophyll, carotenoids, lipids, phenols, reducing sugars, and alkaloids using standard laboratory methods.
The results revealed the presence of appreciable amounts of these biochemical constituents, indicating the rich nutritional and medicinal value of Tulsi. Chlorophyll and carotenoid contents reflected the healthy physiological status of the plant, while phenols and alkaloids highlighted its strong antioxidant and therapeutic potential. The presence of lipids and reducing sugars further demonstrated its metabolic and biochemical importance.
The study concludes that Tulsi is a biochemically rich medicinal plant with significant pharmaceutical and nutritional value. These findings support its traditional use in herbal medicine and provide a basis for future research on phytochemical analysis, drug development, and the formulation of herbal healthcare products.